2D68

Structure of the N-terminal domain of FOP (FGFR1OP) protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-16
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	P 21 21 2
Unit cell lengths	47.840, 92.180, 37.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.20745
Rwork	0.205
R-free	0.24719
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.229
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.080	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	21205
Completeness [%]	94.0	86.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0,2M NaCl; 0,1M HEPES ph 7,5, 25% w/v PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0,2M NaCl; 0,1M HEPES ph 7,5, 25% w/v PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K