2D62

Crystal structure of multiple sugar binding transport ATP-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97243
Spacegroup name	P 61 2 2
Unit cell lengths	119.304, 119.304, 180.503
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.840 - 2.100
R-factor	0.198
Rwork	0.198
R-free	0.21400
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.082
Number of reflections	44713
<I/σ(I)>	10.5
Completeness [%]	99.6	98.6
Redundancy	16.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.5	295	Sodium Citrate, Ammonium sulfate, Lithium sulfate, pH 4.5, MICROBATCH, temperature 295.0K