2D62
Crystal structure of multiple sugar binding transport ATP-binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97243 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 119.304, 119.304, 180.503 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.840 - 2.100 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.21400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 | |
Number of reflections | 44713 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 16.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.5 | 295 | Sodium Citrate, Ammonium sulfate, Lithium sulfate, pH 4.5, MICROBATCH, temperature 295.0K |