2D5H
Crystal Structure of Recombinant Soybean Proglycinin A3B4 subunit, its Comparison with Mature Glycinin A3B4 subunit, Responsible for Hexamer Assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1998-10-16 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.378, 223.738, 88.660 |
Unit cell angles | 90.00, 119.85, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.32500 |
Structure solution method | MIR |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.870 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 68145 | |
Completeness [%] | 89.5 | 40.2 |
Redundancy | 3.7 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 20% MPD, 0.1M MES pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |