2D4Z
Crystal structure of the cytoplasmic domain of the chloride channel ClC-0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.919 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 125.819, 125.819, 125.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.890 - 3.100 |
| R-factor | 0.274 |
| Rwork | 0.274 |
| R-free | 0.30900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.053 | 0.507 |
| Number of reflections | 12275 | |
| <I/σ(I)> | 23 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 3.5M sodium formate 10mM magnesium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
