2D4Y
Crystal structure of a 49K fragment of HAP1 (FlgK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0394 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.928, 99.697, 109.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.540 - 2.100 |
| R-factor | 0.22064 |
| Rwork | 0.219 |
| R-free | 0.25504 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.356 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.325 |
| Number of reflections | 56302 | |
| <I/σ(I)> | 19.2 | 4.6 |
| Completeness [%] | 97.8 | 97.7 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 289 | 14-15% PEG 8000, 20-25% isopropanol, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, temperature 289K |
| 1 | VAPOR DIFFUSION | 5.5 | 289 | 14-15% PEG 8000, 20-25% isopropanol, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, temperature 289K |
