2D4Y
Crystal structure of a 49K fragment of HAP1 (FlgK)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-06 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0394 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.928, 99.697, 109.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.540 - 2.100 |
R-factor | 0.22064 |
Rwork | 0.219 |
R-free | 0.25504 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.356 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.063 | 0.325 |
Number of reflections | 56302 | |
<I/σ(I)> | 19.2 | 4.6 |
Completeness [%] | 97.8 | 97.7 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 289 | 14-15% PEG 8000, 20-25% isopropanol, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, temperature 289K |
1 | VAPOR DIFFUSION | 5.5 | 289 | 14-15% PEG 8000, 20-25% isopropanol, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, temperature 289K |