2D4X
Crystal structure of a 26K fragment of HAP3 (FlgL)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.651, 59.651, 106.383 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.990 - 1.900 |
R-factor | 0.21142 |
Rwork | 0.209 |
R-free | 0.25809 |
Structure solution method | MIR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.456 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.263 |
Number of reflections | 17862 | |
<I/σ(I)> | 28.1 | 9.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.2 | 277 | 10-20% PEG 8000, 0.2M magnesium chloride, 0.1M Phosphate-citrate, pH 4.2, VAPOR DIFFUSION, temperature 277K |