2D4W
Crystal structure of glycerol kinase from Cellulomonas sp. NT3060
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.130 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 105.430, 105.430, 195.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19535 |
| Rwork | 0.193 |
| R-free | 0.24046 |
| Structure solution method | SIROAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.593 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.104 | |
| Number of reflections | 49935 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | 27-37% MPD, 0.1M citrate, pH 4.5, VAPOR DIFFUSION, temperature 277K |
