2D4U
Crystal Structure of the ligand binding domain of the bacterial serine chemoreceptor Tsr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2005-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.480, 55.060, 73.330 |
| Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
| Resolution | 43.850 - 1.950 |
| R-factor | 0.21805 |
| Rwork | 0.216 |
| R-free | 0.25854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lig |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.322 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.056 | 0.219 |
| Number of reflections | 24467 | |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 15-20% PEG 10000, 0.1M Tris HCl, pH 7.5, VAPOR DIFFUSION, temperature 293K |
