2D4R
Crystal structure of TTHA0849 from Thermus thermophilus HB8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-26 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97911, 0.97940, 0.90000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.300, 86.490, 109.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.290 - 2.400 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.29900 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.000 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.280 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 38812 | |
Completeness [%] | 96.1 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 25% PEG4000, 0.2M ammonium sulfate, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |