2D4M
Crystal Structure of apo M-PMV dUTPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8031 |
Spacegroup name | P 63 |
Unit cell lengths | 61.191, 61.191, 63.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.850 |
R-factor | 0.16194 |
Rwork | 0.160 |
R-free | 0.19193 |
Starting model (for MR) | 2d4l |
RMSD bond length | 0.020 |
RMSD bond angle | 1.862 |
Data scaling software | XDS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 1.940 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.091 | 0.503 |
Number of reflections | 11637 | |
<I/σ(I)> | 8.85 | 2.66 |
Completeness [%] | 97.4 | 95.04 |
Redundancy | 2.74 | 2.54 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 8000, AMMONIUM CHLORIDE, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |