2D4I
Monoclinic hen egg-white lysozyme crystallized at pH4.5 form heavy water solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2004-05-07 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.008, 62.915, 60.494 |
| Unit cell angles | 90.00, 90.55, 90.00 |
Refinement procedure
| Resolution | 17.200 - 1.160 |
| R-factor | 0.137 |
| Rwork | 0.136 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lj4 |
| Data reduction software | SaintPlus |
| Data scaling software | SAINTPLUS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.200 | 1.170 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.067 | 0.415 |
| Number of reflections | 68963 | |
| Completeness [%] | 88.7 | 57.3 |
| Redundancy | 2.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 4.5 | 298 | 1% protein, 2% sodium nitrate, D2O solution with pD 4.5 adjusted with 1M acetic acid, batch method, temperature 298K |
