2D4F
The Crystal Structure of human beta2-microglobulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.649, 28.869, 54.756 |
| Unit cell angles | 90.00, 121.66, 90.00 |
Refinement procedure
| Resolution | 26.450 - 1.700 |
| R-factor | 0.20833 |
| Rwork | 0.207 |
| R-free | 0.23339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lds |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.448 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.960 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.069 | 0.412 |
| Number of reflections | 11279 | |
| <I/σ(I)> | 8.1 | 2.4 |
| Completeness [%] | 97.3 | 87.1 |
| Redundancy | 3.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 20% PEG4000, 25% glycerol, 0.2M ammonium acetate, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
