2D4D
The Crystal Structure of human beta2-microglobulin, L39W W60F W95F Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-24 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.423, 28.776, 57.241 |
| Unit cell angles | 90.00, 123.56, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.100 |
| R-factor | 0.21802 |
| Rwork | 0.216 |
| R-free | 0.26543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lds |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.673 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | |
| Number of reflections | 6343 | |
| <I/σ(I)> | 8.2 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 15% PEG4000, 25% glycerol, 0.2M ammonium acetate, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
