2D4D
The Crystal Structure of human beta2-microglobulin, L39W W60F W95F Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-24 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.423, 28.776, 57.241 |
Unit cell angles | 90.00, 123.56, 90.00 |
Refinement procedure
Resolution | 47.670 - 2.100 |
R-factor | 0.21802 |
Rwork | 0.216 |
R-free | 0.26543 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lds |
RMSD bond length | 0.010 |
RMSD bond angle | 1.301 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.673 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | |
Number of reflections | 6343 | |
<I/σ(I)> | 8.2 | 3.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.5 | 927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 15% PEG4000, 25% glycerol, 0.2M ammonium acetate, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K |