2D4A

Structure of the malate dehydrogenase from Aeropyrum pernix

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2004-11-24
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	78.168, 84.012, 216.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.870
R-factor	0.2053
Rwork	0.205
R-free	0.24000
Structure solution method	SIRAS
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.970
High resolution limit [Å]	2.870	2.870
Number of reflections	33305
Completeness [%]	99.8	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	40% PEG600, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K