2D48
Crystal structure of the Interleukin-4 variant T13D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-08-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9178 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 90.528, 90.528, 45.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.980 - 1.650 |
R-factor | 0.22222 |
Rwork | 0.221 |
R-free | 0.24709 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hik |
RMSD bond length | 0.018 |
RMSD bond angle | 1.695 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.600 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.092 | 0.172 |
Number of reflections | 22700 | |
<I/σ(I)> | 17.6 | 5.2 |
Completeness [%] | 97.1 | 98.1 |
Redundancy | 6.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 52% Ammonium sulfate, 0.1M Sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |