2D3V
Crystal Structure of Leukocyte Ig-like Receptor A5 (LILRA5/LIR9/ILT11)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97921, 0.97956, 0.90000, 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.080, 58.645, 131.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.850 |
R-factor | 0.23547 |
Rwork | 0.234 |
R-free | 0.26861 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.551 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.052 | 0.382 |
Number of reflections | 19328 | |
<I/σ(I)> | 16.6 | 2.5 |
Completeness [%] | 92.1 | 49.2 |
Redundancy | 6.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Bicine, PEG20000, Dioxane, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |