2D3T
Fatty Acid beta-oxidation multienzyme complex from Pseudomonas Fragi, Form V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.834 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.227, 137.552, 198.139 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.400 |
R-factor | 0.248 |
Rwork | 0.242 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wdk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.823 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1000.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 35837 | |
Completeness [%] | 97.9 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG4000, MES, N-OCTYLPENTAOXYETHYLENE, NAD+, ACETOACETYL-COA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |