2D3S
Crystal Structure of basic winged bean lectin with Tn-antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.866, 91.897, 73.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.204 |
| Rwork | 0.198 |
| R-free | 0.24400 |
| Starting model (for MR) | PDB CODE 1wbl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.086 | 0.416 |
| Number of reflections | 44561 | |
| <I/σ(I)> | 18.8 | 4.1 |
| Completeness [%] | 97.5 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 6-7% PEG4000,15% Isopropanol, 0.02M PBS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
