2D3O
Structure of Ribosome Binding Domain of the Trigger Factor on the 50S ribosomal subunit from D. radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.500, 410.500, 695.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.840 - 3.350 |
| R-factor | 0.299 |
| Rwork | 0.299 |
| R-free | 0.32200 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 322358 | |
| Completeness [%] | 93.5 | 75.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 290 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ETHANOL | ||
| 10 | 1 | 2 | NH4CL | ||
| 2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | HEPES | ||
| 6 | 1 | 1 | NH4CL | ||
| 7 | 1 | 2 | MGCL2 | ||
| 8 | 1 | 2 | KCL | ||
| 9 | 1 | 2 | HEPES |
