2D3O
Structure of Ribosome Binding Domain of the Trigger Factor on the 50S ribosomal subunit from D. radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9798 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.500, 410.500, 695.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.840 - 3.350 |
R-factor | 0.299 |
Rwork | 0.299 |
R-free | 0.32200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.470 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 322358 | |
Completeness [%] | 93.5 | 75.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 290 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ETHANOL | ||
10 | 1 | 2 | NH4CL | ||
2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | KCL | ||
5 | 1 | 1 | HEPES | ||
6 | 1 | 1 | NH4CL | ||
7 | 1 | 2 | MGCL2 | ||
8 | 1 | 2 | KCL | ||
9 | 1 | 2 | HEPES |