2D3I
Crystal Structure of Aluminum-Bound Ovotransferrin at 2.15 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-07 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.8 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.353, 59.184, 87.974 |
Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
Resolution | 49.030 - 2.150 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ovt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 40021 | |
<I/σ(I)> | 16.5 | 4.2 |
Completeness [%] | 98.4 | 94 |
Redundancy | 5.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | PEG400, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |