2D3D
crystal structure of the RNA binding SAM domain of saccharomyces cerevisiae Vts1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.380, 27.890, 99.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.600 - 1.600 |
| R-factor | 0.20811 |
| Rwork | 0.206 |
| R-free | 0.25571 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.334 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.600 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 9742 | |
| <I/σ(I)> | 26.7 | 5.3 |
| Completeness [%] | 92.4 | 95.9 |
| Redundancy | 5.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 4000, ammonium chloride, calcium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
