2D30
Crystal Structure of Cytidine Deaminase Cdd-2 (BA4525) from Bacillus Anthracis at 2.40A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.792, 50.552, 70.438 |
| Unit cell angles | 90.00, 132.10, 90.00 |
Refinement procedure
| Resolution | 27.890 - 2.400 |
| R-factor | 0.16825 |
| Rwork | 0.164 |
| R-free | 0.25075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 | 0.172 |
| Number of reflections | 9578 | |
| <I/σ(I)> | 15.5 | 5.7 |
| Completeness [%] | 92.7 | 62.1 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG2000 MME, Ca_acetate, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
