2D2Z
Crystal structure of Soluble Form Of CLIC4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.190, 86.050, 73.380 |
| Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
| Resolution | 23.490 - 2.200 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.490 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.365 |
| Number of reflections | 43497 | |
| <I/σ(I)> | 7.8 | 17.7 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | sodium citrate, ethanol, Magnesium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
