2D2X
Crystal structure of 2-deoxy-scyllo-inosose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.510, 70.350, 82.984 |
| Unit cell angles | 90.00, 117.84, 90.00 |
Refinement procedure
| Resolution | 24.770 - 2.300 |
| R-factor | 0.207 |
| Rwork | 0.191 |
| R-free | 0.22800 |
| Structure solution method | MIR |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.770 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 36626 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 277 | 40%(w/v) PEG 4000, 200mM Li2SO4, 100mM Tris-HCl, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
