2D2Q
Crystal structure of the dimerized radixin FERM domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-07 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.218, 70.218, 110.810 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| Rwork | 0.222 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 19703 | |
| Completeness [%] | 96.4 | 76.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG 3350, HEPES-Na, pH 7.5, VAPOR DIFFUSION, temperature 293K |
