2D2L
Crystal Structure of a minimal, all-RNA hairpin ribozyme with a propyl linker (C3) at position U39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.200, 93.200, 122.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.670 - 2.500 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.24900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x9c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.051 | 0.420 |
| Number of reflections | 11302 | |
| <I/σ(I)> | 29.6 | 7 |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 12.9 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000 MME, LITHIUM SULFATE, SPERMIDINE, COBALT HEXAAMINE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG | ||
| 10 | 1 | 2 | water | ||
| 2 | 1 | 1 | LITHIUM SULFATE | ||
| 3 | 1 | 1 | SPERMIDINE | ||
| 4 | 1 | 1 | COBALT HEXAAMINE | ||
| 5 | 1 | 1 | water | ||
| 6 | 1 | 2 | PEG | ||
| 7 | 1 | 2 | LITHIUM SULFATE | ||
| 8 | 1 | 2 | SPERMIDINE | ||
| 9 | 1 | 2 | COBALT HEXAAMINE |
