2D2E
Crystal structure of atypical cytoplasmic ABC-ATPase SufC from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Detector technology | CCD |
| Collection date | 2003-06-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.361, 71.247, 51.178 |
| Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
| Resolution | 31.920 - 1.700 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25099 | |
| <I/σ(I)> | 53.9 | 13.5 |
| Completeness [%] | 99.3 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 0.1M Tris, 15% PEG 4000, 150mM MgCl2, 20% glycerol, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
