2D28

Structure of the N-terminal domain of XpsE (crystal form P43212)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-11
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9537, 0.97990, 0.97980
Spacegroup name	P 43 21 2
Unit cell lengths	55.914, 55.914, 104.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 2.000
R-factor	0.192
Rwork	0.189
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.022
RMSD bond angle	2.175
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.045	0.090
Number of reflections	12460
Completeness [%]	99.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	magnesium acetate, sodium cacodylate pH 6.5, PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 277K