2D27

Structure of the N-terminal domain of XpsE (crystal form I4122)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL12B2
Synchrotron site	SPring-8
Beamline	BL12B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9537, 0.9801, 0.9796
Spacegroup name	I 41 2 2
Unit cell lengths	92.479, 92.479, 123.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 2.210
R-factor	0.195
Rwork	0.193
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.024
RMSD bond angle	2.055
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.290
High resolution limit [Å]	2.210	2.210
Number of reflections	13800
Completeness [%]		99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	ammonium sulfate, MgCl2, MES pH 5.6, PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K