2D25
C-C-A-G-G-C-M5C-T-G-G; HELICAL FINE STRUCTURE, HYDRATION, AND COMPARISON WITH C-C-A-G-G-C-C-T-G-G
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 6 |
| Unit cell lengths | 53.770, 53.770, 34.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.750 |
| R-factor | 0.174 |
| RMSD bond length | 0.045 |
| RMSD bond angle | 0.064 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 1.750 |
| Rmerge | 0.038 * |
| Number of reflections | 4704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 4 * | referred to 'Heinemann, U.', (1991) 'Embo J.', 10, 35-43 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | DNA solution | 2 (mM) | ||
| 2 | 1 | sodium cacodylate | 20 (mM) | ||
| 3 | 1 | magnesium chloride | 50 (mM) | ||
| 4 | 1 | 2 | MPD | 40 (%) | |
| 5 | 1 | 2 | sodium cacodylate | 20 (mM) | |
| 6 | 1 | 2 | magnesium chloride | 50 (mM) |
