2D1W
Substrate Schiff-Base intermediate with tyramine in copper amine oxidase from Arthrobacter globiformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-11 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 157.570, 63.351, 183.651 |
| Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
| Resolution | 26.444 - 1.740 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.500 | 1.830 |
| High resolution limit [Å] | 1.600 | 1.740 |
| Number of reflections | 171652 | |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.8 | 289 | 1.05M potassium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |
