2D1V
Crystal structure of DNA-binding domain of Bacillus subtilis YycF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-07 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.754, 65.584, 79.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.228 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.103 | 0.377 |
| Number of reflections | 4659 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 95.2 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.6 | 289 | 90mM HEPES, 9mM MgCl2, 3% dioxane, pH 6.6, MICRODIALYSIS, temperature 289K |
