2D1Q
Crystal structure of the thermostable Japanese Firefly Luciferase complexed with MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-16 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.780, 181.975, 53.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.300 |
| R-factor | 0.18151 |
| Rwork | 0.179 |
| R-free | 0.22766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lci |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.058 | 0.150 |
| Number of reflections | 25394 | |
| <I/σ(I)> | 10.7 | 4 |
| Completeness [%] | 97.9 | 91.1 |
| Redundancy | 3.1 | 2.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | PEG4000, Lithium chrolide, ATP, magnesium chloride, Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 293K |
