2D16

Crystal Structure of PH1918 protein from Pyrococcus horikoshii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Detector technology	IMAGE PLATE
Collection date	2005-07-20
Detector	RIGAKU
Wavelength(s)	0.9
Spacegroup name	P 3
Unit cell lengths	66.120, 66.120, 125.072
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.700 - 1.650
R-factor	0.185
Rwork	0.185
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vgg
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.066
Number of reflections	73383
<I/σ(I)>	7.1
Completeness [%]	100.0	100
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.3	295	MES-NaOH, PEG 4000, pH 6.3, microbatch, temperature 295.0K