2D13
Crystal Structure of PH1257 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-20 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.974, 96.696, 84.921 |
| Unit cell angles | 90.00, 93.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RU8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | |
| Number of reflections | 32935 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.2 | 295 | Tris-HCl, CaCl2, PEG4000, pH 8.2, microbatch, temperature 295.0K |
