2D0W
Crystal structure of cytochrome cL from Hyphomicrobium denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.7 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.779, 57.283, 67.007 |
Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
Resolution | 29.240 - 1.980 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.240 | 2.070 |
High resolution limit [Å] | 1.980 | 2.000 |
Number of reflections | 22502 | |
Completeness [%] | 95.0 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 289 | PEG10000, zinc sulfate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K |