2D0J
Crystal Structure of Human GlcAT-S Apo Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.402, 122.591, 92.985 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.2395 |
| Rwork | 0.237 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v82 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.049 | 0.258 |
| Number of reflections | 76289 | |
| <I/σ(I)> | 23.7 | 6.9 |
| Completeness [%] | 96.0 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | PEG5000 MME, di-sodium hydrogen phosphate, cesium chloride, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
