2D0C

Crystal structure of Bst-RNase HIII in complex with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Detector technology	CCD
Collection date	2004-11-10
Detector	Bruker DIP-6040
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	67.288, 109.771, 48.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.640 - 2.600
R-factor	0.209
Rwork	0.209
R-free	0.27400
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.100
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	11062

Crystallization Conditions

crystal ID	method	pH	temperature	details
1