2CZV
Crystal structure of archeal RNase P protein ph1481p in complex with ph1877p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.332, 76.118, 151.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.690 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26100 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 49669 | |
Completeness [%] | 99.0 | 96.8 |
Redundancy | 6.3 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, magnesium acetate, n-octly b-glucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 3350, magnesium acetate, n-octly b-glucoside, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |