2CZR

Crystal structure of TBP-interacting protein (Tk-TIP26) and implications for its inhibition mechanism of the interaction between TBP and TATA-DNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793, 0.9795, 0.9873
Spacegroup name	P 43 21 2
Unit cell lengths	73.834, 73.834, 86.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.920 - 2.300
R-factor	0.191
Rwork	0.191
R-free	0.23100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.420
High resolution limit [Å]	2.300	2.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	PEG8000, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	PEG8000, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	PEG8000, MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K