2CZQ

A novel cutinase-like protein from Cryptococcus sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	34.478, 82.807, 123.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.050
R-factor	0.14843
Rwork	0.147
R-free	0.15716
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.167
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	1.110
High resolution limit [Å]	1.050	1.050
Rmerge	0.071	0.380
Number of reflections	157936
<I/σ(I)>	7.4	2.2
Completeness [%]	95.4	95.4
Redundancy	7.1	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.9	277	1.3-1.6M Sodium citrate, pH 5.9, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	5.9	277	1.3-1.6M Sodium citrate, pH 5.9, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	5.9	277	1.3-1.6M Sodium citrate, pH 5.9, VAPOR DIFFUSION, temperature 277K