2CZJ

Crystal structure of the tRNA domain of tmRNA from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	84.776, 67.957, 178.662
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	48.760 - 3.010
Rwork	0.255
R-free	0.32000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p6v 1wjx
RMSD bond length	0.014
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	3.110
High resolution limit [Å]	3.010	3.010
Number of reflections	40370
<I/σ(I)>	23.69	3.5
Completeness [%]	99.5	98.6
Redundancy	6.33	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	HEPES
2	1	1	ammonium sulfate
3	1	2	ammonium sulfate