2CZI
Crystal structure of human myo-inositol monophosphatase 2 (IMPA2) with calcium and phosphate ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 106.295, 106.295, 54.857 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.130 - 3.000 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2czh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 7387 | |
| <I/σ(I)> | 12.3 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.008M Potassium dihydrogen phosphate, 14% PEG 8000, 20% Glycerol, 5mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
