2CZ9
Crystal Structure of galactokinase from Pyrococcus horikoshi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-17 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.95 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.081, 38.905, 81.566 |
| Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
| Resolution | 28.140 - 1.500 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s4e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.260 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.054 | 0.464 |
| Number of reflections | 86789 | |
| <I/σ(I)> | 18.9 | 2.1 |
| Completeness [%] | 97.4 | 94.2 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.3 | 291 | PEG4000, magnesium chloride, MES, pH 6.3, Micro batch, temperature 291K |
