2CZ7

Fe-type NHase photo-activated for 75min at 105K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	105
Detector technology	CCD
Collection date	2001-10-29
Detector	MAR CCD 165 mm
Wavelength(s)	0.7
Spacegroup name	C 1 2 1
Unit cell lengths	114.798, 60.384, 81.804
Unit cell angles	90.00, 124.98, 90.00

Refinement procedure

Resolution	19.770 - 1.800
R-factor	0.143
Rwork	0.143
R-free	0.15300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	42845
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG8000, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K