2CZ4
Crystal structure of a putative PII-like signaling protein (TTHA0516) from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-11 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97889, 0.97939, 0.96000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.345, 117.699, 114.114 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.110 - 1.930 |
| Rwork | 0.192 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 30273 | |
| <I/σ(I)> | 18.9 | 4.9 |
| Completeness [%] | 96.6 | 77.9 |
| Redundancy | 7.7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 0.1M Sodium acetate, 1.75M NaCl, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
