2CY9
Crystal structure of thioesterase superfamily member2 from Mus musculus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-20 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97922, 0.97957, 0.964 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.522, 90.195, 61.210 |
| Unit cell angles | 90.00, 118.32, 90.00 |
Refinement procedure
| Resolution | 49.100 - 2.720 |
| R-factor | 0.19404 |
| Rwork | 0.191 |
| R-free | 0.26243 |
| Structure solution method | MAD |
| RMSD bond length | 0.078 |
| RMSD bond angle | 4.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 9667 | |
| <I/σ(I)> | 8.58 | 2.9 |
| Completeness [%] | 71.0 | 23.8 |
| Redundancy | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris-HCl, 2.0 M ammonium sulphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
