2CY6
Crystal structure of ConM in complex with trehalose and maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.4270 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.033, 97.332, 71.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.960 - 2.000 |
| R-factor | 0.18742 |
| Rwork | 0.185 |
| R-free | 0.23909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.049 |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.578 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32114 | |
| Completeness [%] | 98.4 | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP, SOAKING | 7.5 | 293 | Hepes-Na, ammonium sulfate, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, SOAKING, temperature 293.0K |
