2CY5

Crystal structure of phosphotyrosine binding (PTB) domain of epidermal growth factor receptor pathway substrate-8 (EPS8) related protein 1 from Mus musculus (form-2 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-07
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	73.385, 73.385, 59.889
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 1.900
R-factor	0.206
Rwork	0.206
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.036
RMSD bond angle	2.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	14823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG400, HEPES, Tris, calcium chloride, sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K