2CXY
Crystal structure of the hBAF250b AT-rich interaction domain (ARID)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-13 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9791, 0.9794, 0.9640 |
| Spacegroup name | P 65 |
| Unit cell lengths | 68.870, 68.870, 46.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.530 - 1.600 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.20300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.580 |
| Rmerge | 0.067 | 0.346 |
| Number of reflections | 16439 | |
| <I/σ(I)> | 28.2 | 3.34 |
| Completeness [%] | 100.0 | 67.6 |
| Redundancy | 13 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | tri-sodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
